Homefoil for nucleic acid analysis based on isothermal recombina
Miniaturized devices for isothermal DNA amplification ...
Dec 21, 2015· Lutz S et al (2010) Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA). Lab Chip 10:887–893. doi:10.1039/b921140c. Article Google Scholar
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Lab-on-a-chip solutions designed for being operated on ...
[5] Lutz S, Weber P, Focke M, Faltin B, Hoffmann J, Müller C et al. Microfluidic Lab-on-a-Foil for Nucleic Acid Analysis based on Isothermal Recombinase Polymerase Amplification of DNA. Lab Chip 2009; 10 :887-93. [6] Burger J, Mark D, Roth G, Müller C, Zengerle R, von Stetten F, Park Y. Physiologische Assays in einem DVD Player am Beispiel der
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1. Introduction
Other technologies employing a constant temperature to facilitate the amplification of nucleic acids targets have also been reported in the literature. In contrast to PCR, the use of these isothermal technologies reduces the need for sophisticated instrumentation, consistent electrical power supply, and complex sample processing protocols [15, 16].
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Insights and Prediction of Isothermal Nucleic Acid ...
The newly Isothermal Nucleic Acid Amplification market report provided a complete view of the present and future market. Deep analysis of enterprise competition pattern, enterprise product advantages and disadvantages, regional industrial layout characteristics. The base year considered for the study is 2018 and the market size is projected from 2019 to 2026.
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Isothermal amplification methods for the detection of ...
Dec 27, 2012· 1. Introduction. A great effort has been devoted, during the past decade, to the development of new devices for specific DNA sequences detection, due to the relevance of nucleic acid analysis in many areas, including clinical diagnostics [], environmental monitoring and food-quality control [2,3].However, diagnostic tools used to detect nucleic acids need to meet specific .
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Rapid detection of Plasmodium falciparum with isothermal ...
Mar 15, 2014· Nucleic acid amplification is the most sensitive and specific method to detect Plasmodium falciparum. However the polymerase chain reaction remains laboratory-based and has to be conducted by trained personnel. Furthermore, the power dependency for the thermocycling process and the costly equipment necessary for the read-out are difficult to cover in resource-limited settings.
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Peer-reviewed RPA publications | TwistDx
Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA). Author: Lutz S, Weber P, Focke M, Faltin B, Hoffmann J, Müller C, Mark D, Roth G, Munday P, Armes N,Piepenburg O, Zengerle R, von Stetten F.
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Ultrasensitive CRISPR-based diagnostic for field ...
Oct 13, 2020· The furthest developed commercial nucleic acid amplification tests (NAAT) for malaria are loop-mediated isothermal amplification-based assays, but they have exhibited disappointing sensitivity in field studies in comparison to PCR and require separate nucleic acid extraction steps (10 ⇓ .
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Recombinase Polymerase Amplification: A New DNA/RNA ...
Cite this article: GAO Wei-Fang,ZHU Peng,HUANG Hai-Long. Recombinase Polymerase Amplification: A New DNA/RNA Amplification Strategy[J]. Chinese Journal of .
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(PDF) Lab-on-a-Foil: microfluidics on thin and flexible ...
In the second part, an analysis of the state of Lab-on-a-Foil applications is provided with a special focus on nucleic acid analysis, immunoassays, cell-based assays and home care testing. We conclude that the Lab-on-a-Foil approach is very versatile and significantly expands the toolbox for the development of Lab-on-a-Chip solutions.
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SARS-CoV-2 detection using isothermal amplification and a ...
Sep 29, 2020· This work describes the optimization of a sample preparation and detection pipeline for a SARS-CoV-2 diagnostic test that is rapid and does not require specialized equipment. This pipeline consists of viral inactivation, rendering samples safer to work with, followed by a sensitive 30-min isothermal detection reaction with a color-based red to yellow readout.
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Multiplexed Isothermal Amplification Based Diagnostic ...
Therefore, nucleic acid testing remains the most reliable way to detect and differentiate these viruses. Detection of Zika and other mosquito-borne viruses is usually performed using RT-PCR or real-time RT-PCR in variety of biological fluids, such as serum, urine, saliva, semen, breast milk, and cerebral fluid 10, .
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Recombinase Polymerase Amplification: A New DNA/RNA ...
Cite this article: GAO Wei-Fang,ZHU Peng,HUANG Hai-Long. Recombinase Polymerase Amplification: A New DNA/RNA Amplification Strategy[J]. Chinese Journal of .
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Lab on a Chip Dynamic Article Links - OptiGene
Isothermal nucleic acid amplification technologies for point-of-care diagnostics: a critical review Pascal Craw* and Wamadeva Balachandran Received 27th January 2012, Accepted 26th March 2012 DOI: 10.1039/c2lc40100b Nucleic Acid Testing (NAT) promises rapid, sensitive and specific diagnosis of infectious, inherited and genetic disease.
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Isothermal DNA amplification in bioanalysis: strategies ...
Jan 20, 2011· 78 Lutz S, Weber P, Focke M et al. Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA). Lab Chip 10(7),887–893 ( 2010 ). Crossref, Medline, CAS, Google Scholar
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US9738889B2 - Rapid nucleic acid extraction method and ...
nucleic acid syringe capsule membrane solution Prior art date 2014-10-17 Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Active, expires 2035-01-03 Application number US14/830,210 Other versions ...
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Digital Isothermal Quantification of Nucleic Acids via ...
isothermal quantification of nucleic acids in a sequence-specific manner by using recombinase polymerase amplification (RPA). Quantitative analysis of nucleic acids is important for studying geneexpression1 andmoleculardiagnostics,suchasfordetection of pathogens,2 4 analysis of genomic diseases and cancer,5,6
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Microfluidic lab-on-a-foil for nucleic acid analysis based ...
Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA) Sascha Lutz,†*a Patrick Weber,†a Max Focke,b Bernd Faltin,b Jochen Hoffmann,b Claas M€uller, c Daniel Mark,a G€unter Roth, b Peter Munday,d Niall Armes,d Olaf Piepenburg,d Roland Zengerleabe and Felix von Stettenab Received 8th October 2009, Accepted 11th December 2009
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Peer-reviewed RPA publications | TwistDx
Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA). Author: Lutz S, Weber P, Focke M, Faltin B, Hoffmann J, Müller C, Mark D, Roth G, Munday P, Armes N,Piepenburg O, Zengerle R, von Stetten F.
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Isothermal DNA amplification in bioanalysis: strategies ...
Jan 20, 2011· 78 Lutz S, Weber P, Focke M et al. Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA). Lab Chip 10(7),887–893 ( 2010 ). Crossref, Medline, CAS, Google Scholar
Get priceCCIS 318 - The LabDisk – A Fully Automated Centrifugal Lab ...
plement a nucleic acid analysis and an immunoassay. For both assays, the complete microfluidic structures have been integrated into the foil-based cartridges and all the microfluidic unit operations required to perform the assays have been verified. Nucleic Acid Analysis
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Digital Isothermal Quantification of Nucleic Acids via ...
In this paper, digital quantitative detection of nucleic acids was achieved at the single-molecule level by chemical initiation of over one thousand sequence-specific, nanoliter isothermal amplification reactions in parallel. Digital polymerase chain reaction (digital PCR), a method used for quantification of nucleic acids, counts the presence or absence of amplification of individual ...
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[PDF] Multiplex isothermal solid-phase recombinase ...
AbstractWe report on the development of an on-chip RPA (recombinase polymerase amplification) with simultaneous multiplex isothermal amplification and detection on a solid surface. The isothermal RPA was applied to amplify specific target sequences from the pathogens Neisseria gonorrhoeae, Salmonella enterica and methicillin-resistant Staphylococcus aureus (MRSA) using genomic DNA.
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(PDF) Isothermal Polymerase Amplification in a Centrifugal ...
For the first time we demonstrate a self-sufficient lab-on-a-foil system for the fully automated analysis of nucleic acids which is based on the recently available isothermal recombinase ...
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Isothermal DNA amplification in bioanalysis: strategies ...
Jan 20, 2011· 78 Lutz S, Weber P, Focke M et al. Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA). Lab Chip 10(7),887–893 ( 2010 ). Crossref, Medline, CAS, Google Scholar
Get priceMultiplexed Isothermal Amplification Based Diagnostic ...
Therefore, nucleic acid testing remains the most reliable way to detect and differentiate these viruses. Detection of Zika and other mosquito-borne viruses is usually performed using RT-PCR or real-time RT-PCR in variety of biological fluids, such as serum, urine, saliva, semen, breast milk, and cerebral fluid 10, .
Get priceMicrofluidics: innovative approaches for rapid diagnosis ...
For on-site analysis, sample pre-processing is often required in order to obtain nucleic acids that are suitable for microfluidic analysis . Various methods such as exposure to heat, lytic enzymes and different kinds of chemicals have been utilized to facilitate cell disruption in order to extract the DNA from the specimen [ 38, 40, 42, 43 ].
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Multiplex isothermal solid-phase recombinase polymerase ...
Feb 18, 2014· Lutz S, Weber P, Focke M, Faltin B, Hoffmann J, Müller C, Mark D, 27. Andresen D, von Nickisch-Rosenegk M, Bier FF (2009) Helicase Roth G, Munday P, Armes N, Piepenburg O, Zengerle R, von Stetten dependent OnChip-amplification and its use in multiplex pathogen F (2010) Microfluidic lab-on-a-foil for nucleic acid analysis based on detection.
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Point-of-care nucleic acid testing for infectious diseases ...
Nucleic acid testing for infectious diseases at the point of care is beginning to enter clinical practice in developed and developing countries; especially for applications requiring fast turnaround times, and in settings where a centralized laboratory approach faces limitations. Current systems for clinical diagnostic applications are mainly PCR-based, can only be used in hospitals, and are ...
Get price[PDF] Multiplex isothermal solid-phase recombinase ...
AbstractWe report on the development of an on-chip RPA (recombinase polymerase amplification) with simultaneous multiplex isothermal amplification and detection on a solid surface. The isothermal RPA was applied to amplify specific target sequences from the pathogens Neisseria gonorrhoeae, Salmonella enterica and methicillin-resistant Staphylococcus aureus (MRSA) using genomic DNA.
Get price
Web COBIOPHAD MAY 19
For the first time we demonstrate a self-sufficient lab-on-a-foil system for the fully automated analysis of nucleic acids which is based on the recently available isothermal recombinase polymerase amplification (RPA). The system consists
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